2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域

doi:10.11843/j.issn.0366-6964.2016.09.013

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (9): 1848-1852.doi: 10.11843/j.issn.0366-6964.2016.09.013

2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域

杨文志¹，张明月¹，王冠楠¹，赵宇鹏²，张巍巍¹，李世杰^1*

(1.河北农业大学生命科学学院，保定 071001；2.河北北方学院，张家口 075000)

收稿日期:2016-04-05 出版日期:2016-09-23 发布日期:2016-09-23
通讯作者: 李世杰，教授，博士生导师，Tel：0312-7528270，E-mail：lishijie20005@163.com
作者简介:杨文志（1991-），女，河北衡水人，硕士生，主要从事动物分子遗传学研究，Tel：0312-7528270，E-mail：anshiyangwenzhi@163.com
基金资助:
国家自然科学基金项目（31372312；30972098）；河北省自然科学基金项目（C2016204092）

Two Imprinted Long Non-coding RNAs Located in Cattle Dlk1-Dio3 Domain

YANG Wen-zhi¹，ZHANG Ming-yue¹，WANG Guan-nan¹，ZHAO Yu-peng ²，ZHANG Wei-wei ¹，LI Shi-jie^1*

(1.College of Life Science，Agricultural University of Hebei，Baoding 071001，China；2. Hebei North University，Zhangjiakou 075000，China)

Received:2016-04-05 Online:2016-09-23 Published:2016-09-23

摘要/Abstract

摘要：

旨在鉴别牛Dlk1-Dio3印记区域内的新印记lncRNAs。本研究选取位于Meg8与Meg9基因间的2组表达序列标签（EST），通过RT-PCR克隆序列并进行分析，结果发现其具有lncRNA的特征，命名为Linc24062和Linc24063。分析Linc24062和Linc24063在牛8个组织（心、肝、脾、肺、肾、骨骼肌、皮下脂肪和大脑）中的表达，发现2个lncRNAs在被检测的组织中均表达。利用基于单核苷酸多态性（SNP）位点的直接测序法，通过比较杂合子牛中SNP位点处基因组PCR扩增产物与RT-PCR扩增产物的测序峰图分析Linc24062和Linc24063的印记状态，发现Linc24062和Linc24063在牛被检测组织中均为单等位基因表达，说明Linc24062和Linc24063在牛中是印记的。

Abstract:

The objective of this study was to find novel imprinted long noncoding RNAs (lncRNAs) in cattle Dlk1-Dio3 domain.Transcripts mapping to this region between Meg8 and Meg9 were under a systematic search and 2 novel lncRNAs were identified，which were named as Linc24062 and Linc24063 according to annotated bibliography of genecode.The expression patterns of Linc24062 and Linc24063 in adult cattle tissues were characterized using reverse transcription polymerase chain reaction (RT-PCR).The results indicated that Linc24062 and Linc24063 were expressed in all 8 tested tissues，including heart，liver，spleen，lung，kidney，subcutaneous fat，skeletal muscle and brain.The imprinting status of Linc24062 and Linc24063 was assessed based on a polymorphism-based sequencing approach.The results showed that Linc24062 and Linc24063 exhibited monoallelic expression in all examined cattle tissues by comparing sequence chromatograms between genomic DNA and cDNA levels at single nucleotide polymorphism (SNP) site，suggesting that Linc24062 and Linc24063 were imprinted in cattle.

中图分类号:

杨文志，张明月，王冠楠，赵宇鹏，张巍巍，李世杰. 2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域[J]. 畜牧兽医学报, 2016, 47(9): 1848-1852.

YANG Wen-zhi，ZHANG Ming-yue，WANG Guan-nan，ZHAO Yu-peng，ZHANG Wei-wei，LI Shi-jie. Two Imprinted Long Non-coding RNAs Located in Cattle Dlk1-Dio3 Domain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1848-1852.

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2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域

Two Imprinted Long Non-coding RNAs Located in Cattle Dlk1-Dio3 Domain

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